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Paired box 7 inhibits differentiation in 3T3‐L1 preadipocytes
Author(s) -
Izumi Wakana,
Takuma Yuko,
Ebihara Ryo,
Mizunoya Wataru,
Tatsumi Ryuichi,
Nakamura Mako
Publication year - 2018
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.13050
Subject(s) - myf5 , myogenesis , myod , adipogenesis , microbiology and biotechnology , myocyte , 3t3 l1 , myogenic regulatory factors , cellular differentiation , myogenin , biology , stem cell , chemistry , gene , biochemistry , mesenchymal stem cell
Myogenesis is precisely proceeded by myogenic regulatory factors. Myogenic stem cells are activated, proliferated and fused into a multinuclear myofiber. Pax7, paired box 7, one of the earliest markers during myogenesis. It has been reported that Pax7 regulates the muscle marker genes, Myf5 and MyoD toward differentiation. The possible roles of Pax7 in myogenic cells have been well researched. However, it has not yet been clarified if Pax7 itself is able to induce myogenic fate in nonmyogenic lineage cells. In this study, we performed experiments using stably expressed Pax7 in 3T3‐L1 preadipocytes to elucidate if Pax7 inhibits adipogenesis. We found that Pax7 represses adipogenic markers and prevents differentiation. These cells showed decreased expression of PDGFRα, PPARγ and Fabp4 and inhibited forming lipid droplets.