Premium
Skeletal muscle and liver gene expression profiles in finishing steers supplemented with Amaize
Author(s) -
Elolimy Ahmed A.,
Moisá Sonia J.,
Brennan Kristen M.,
Smith Allison C.,
Graugnard Daniel,
Shike Daniel W.,
Loor Juan J.
Publication year - 2018
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.13041
Subject(s) - skeletal muscle , gene , gene expression , biology , anatomy , genetics
Our main objective was to evaluate the effects of feeding α‐amylase (Amaize, Alltech Inc., Nicholasville, KY , USA ) for 140 days on skeletal muscle and liver gene transcription in beef steers. Steers fed Amaize had lower average daily gain ( p = .03) and gain:feed ratio ( p = .05). No differences ( p > .10) in serum metabolites or carcass traits were detected between the two groups but Amaize steers tended ( p < .15) to have increased 12th rib fat depth. Microarray analysis of skeletal muscle revealed 21 differentially expressed genes ( DEG ), where 14 were up‐regulated and seven were down‐regulated in Amaize‐fed steers. The bioinformatics analysis indicated that metabolic pathways involved in fat formation and deposition, stress response, and muscle function were activated, while myogenesis was inhibited in Amaize‐fed steers. The quantitative PCR results for liver revealed a decrease ( p < .01) in expression of fatty acid binding protein 1 ( FABP 1 ) and 3‐hydroxybutyrate dehydrogenase 1 ( BDH 1 ) with Amaize. Because these genes are key for intracellular fatty acid transport, oxidation and ketone body production, data suggest a reduction in hepatic lipid catabolism. Future work to investigate potential positive effects of Amaize on cellular stress response, muscle function, and liver function in beef cattle appears warranted.