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Gene expression profiles of CD 4/ CD 8 double‐positive T cells in porcine peripheral blood
Author(s) -
Okutani Mie,
Tsukahara Takamitsu,
Kato Yoshihiro,
Fukuta Kikuto,
Inoue Ryo
Publication year - 2018
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.13021
Subject(s) - il 2 receptor , effector , biology , antigen , ccl5 , phenotype , microbiology and biotechnology , cd8 , peripheral blood mononuclear cell , cytotoxic t cell , in vitro , immunology , t cell , gene , immune system , genetics
A characteristic subset of T cells, known as double positive T cells ( DPTC ) and expressing both cluster of differentiation 4 ( CD 4) and CD 8, is observed in porcine peripheral blood. Previous studies suggested that DPTC might be memory cells. However, detailed phenotypes and functions of DPTC are yet to be fully elucidated and thus, the relatedness of DPTC with memory phenotypes remains unclear. In this study, DPTC gene expression profiles in peripheral blood were analyzed by DNA microarray in Experiment 1 and compared with those of CD 4 single positive T cells (4 SPTC ) and CD 8 single positive T cells (8 SPTC ). Expressions of IFNG , CCL 5 , NCK 2 , CCR 2 and ITGB 1 were higher than that of 4 SPTC and 8 SPTC . In contrast, expressions of CCR 7 and SELL were lower than that of 4 SPTC and 8 SPTC . These results suggested that DPTC were either effector T cells or effector memory T cells ( T EM ). Next, to determine whether DPTC were effector T cells or T EM , differences in the response of DPTC and 8 SPTC against immunized/primed antigens were compared (Experiment 2). While DPTC showed quick elevation of IL 2 and CD 25 gene expressions against in vitro stimulation of primed/immunized antigens, 8 SPTC did not. These results suggest that at least some DPTC likely belong to T EM .