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Effect of FH 535 on in vitro maturation of porcine oocytes by inhibiting WNT signaling pathway
Author(s) -
Shi Meihong,
Cheng Jianyong,
He Yamei,
Jiang Zhongliang,
Bodinga Bello M.,
Liu Boyang,
Chen Huali,
Li Qingwang
Publication year - 2018
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12982
Subject(s) - wnt signaling pathway , oocyte , in vitro maturation , in vitro , microbiology and biotechnology , biology , signal transduction , cell , apoptosis , andrology , chemistry , embryo , biochemistry , medicine
Abstract Wingless‐int ( WNT ) signaling pathway is vital to modulate life processes, including cell fate determination, cell differentiation, cell proliferation, cell apoptosis and embryogenesis. To demonstrate the uncertain effect of the canonical WNT signaling pathway on oocyte maturation, immature porcine oocytes were collected and cultured in vitro with the WNT /β‐catenin inhibitor FH 535. The concentrations of FH 535 were selected as 0.00, 0.01, 0.10, 1.00 and 10.00 μmol/L. The results showed that the optimum concentration of FH 535 on oocyte maturation was 1.00 μmol/L. In this concentration, the proportion of MII oocytes increased ( P < 0.05). The rate of cleavage was the same with the control ( P > 0.05), while the rate of blastocysts in the 1.00 μmol/L FH 535 treated group was higher than that of control ( P < 0.01). Additionally, the average number of nuclei in blastocysts raised significantly ( P < 0.05). The inhibition of WNT could regulate expression of maturation‐related genes, including Cdc‐2 , Bmp‐15 , Gdf‐9 and Mos . In the 1.00 μmol/L FH 535 treated group, the messenger RNA level of β ‐catenin showed no significant change compared to the control ( P > 0.05), but the protein abundance was decreased ( P < 0.05). This study revealed that the inhibition of FH 535 on the WNT signaling pathway could promote the maturation of porcine oocytes and altered gene expressions in vitro .