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Inclusion of Bacillus amyloliquefaciens strain TOA 5001 in the diet of broilers suppresses the symptoms of coccidiosis by modulating intestinal microbiota
Author(s) -
Tsukahara Takamitsu,
Inoue Ryo,
Nakayama Keizo,
Inatomi Takio
Publication year - 2018
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12980
Subject(s) - coccidiosis , eimeria , eimeria maxima , clostridium perfringens , microbiology and biotechnology , biology , coccidia , broiler , faecalibacterium prausnitzii , colonization , lachnospiraceae , bacillus amyloliquefaciens , feces , veterinary medicine , bacteria , zoology , medicine , parasite hosting , genetics , world wide web , computer science , firmicutes , 16s ribosomal rna
Abstract Coccidiosis is an intestinal parasitic infection and one of the most prevalent and economically damaging diseases of chickens. Furthermore, coccidia‐induced mucogenesis promotes secondary colonization by Clostridium perfringens , a major pathogen of chickens that causes necrotic enteritis. Our previous work found that supernatant of a culture of Bacillus amyloliquefaciens strain TOA 5001 ( BA ) inhibited the growth of C. perfringens on Gifu anaerobic broth medium. Accordingly, we evaluated the effectiveness of dietary BA administration in inhibiting C. perfringens colonization of the intestine in broilers that were experimentally infected with coccidia. Ten healthy broilers from a BA ‐supplemented (2 × 10 5 colony‐forming units/g of feed) broiler group and 10 from a non‐treated group were challenged with Eimeria tenella and E. maxima (5000 oocysts of each species/chick) at 28 days old. At 36 days old, five chicks from each group were slaughtered, whereas the remaining five in each group were killed at 49 days old. Dietary BA administration into Eimeria ‐challenged birds reduced coccidial symptoms such as intestinal lesions. It also modified the cecal microbiota through suppressing C. perfringens and E. coli colonization, and inducing domination of Faecalibacterium prausnitzii , the Lactobacillus group and unknown Lachnospiraceae genera by bacterial DNA ‐based metagenome analyses. B. amyloliquefaciens TOA 5001 supplementation suppressed the symptoms of coccidiosis by modulating cecal microbiota in Eimeria ‐challenged broilers.