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Thyroid hormones alter estrous cyclicity and antioxidative status in the ovaries of rats
Author(s) -
Wei Quanwei,
Fedail Jaafar Sulieman,
Kong Lingfa,
Zheng Kaizhi,
Meng Chunhua,
Fadlalla Mohamed Babo,
Shi Fangxiong
Publication year - 2018
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12950
Subject(s) - medicine , endocrinology , hormone , estrous cycle , luteinizing hormone , glutathione peroxidase , thyroid , ovary , follicle stimulating hormone , antral follicle , biology , malondialdehyde , superoxide dismutase , oxidative stress
To expand our understanding of the roles of thyroid hormones on female reproduction, we induced hypo‐ and hyper‐T rat models to investigate the roles of thyroid hormones on estrous cyclicity, as well as the antioxidative status in the ovaries of rats. In the current study, our data show that hypothyroidism (hypo‐T) and hyperthyroidism (hyper‐T) led to significantly reduced body weights and ovarain weights and delayed vaginal opening day. For hyper‐T, thyroxine (T4), tri‐iodothyronine (T3), progesterone (P4) and follicle‐stimulating hormone ( FSH ) were significantly increased, while estradiol (E2) and luteinizing hormone ( LH ) were significantly decreased. For hypo‐T rats, serum levels of total T4 and T3, E2, P4, FSH and LH were significantly increased, while concentrations of E2 and LH were significantly decreased. For ovary morphology, the numbers of secondary and antral follicles were significantly decreased with more atretic antral follicles and less corpora lutea in both hyper‐ and hypo‐T groups. Both hyper‐T and hypo‐T treatment significantly decreased the expressions of thyroid hormone receptor α 1 in the ovary. Hypo‐T significantly reduced nitric oxide ( NO ), total NO synthase ( tNOS ), inducible NOS and constitutive NOS activities, but hyper‐T increased them. For antioxidative parameters, hypo‐T and hyper‐T treatment significantly increased malondialdehyde ( MDA ) contents. The activities of both glutathione peroxidase ( GSH ‐Px) and catalase ( CAT ) significantly decreased in the hypo‐T group but increased in the hyper‐T group. Total superoxide dismutase (T‐ SOD ) activity was significantly increased in the hyper‐T group. In summary, thyroid hormones alter estrous cyclicity and antioxidative status in the ovary of the rat may act through the NOS signaling pathway.