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Expression levels of taste‐related genes in palate and tongue tip, and involvement of transient receptor potential subfamily M member 5 ( TRPM 5) in taste sense in chickens
Author(s) -
Yoshida Yuta,
Kawabata Fuminori,
Kawabata Yuko,
Nishimura Shotaro,
Tabata Shoji
Publication year - 2018
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12945
Subject(s) - taste , tongue , subfamily , transduction (biophysics) , taste receptor , transient receptor potential channel , biology , gene , microbiology and biotechnology , receptor , genetics , food science , medicine , biochemistry , pathology
The elucidation of the mechanisms underlying the taste sense of chickens will contribute to improvements in poultry feeding, because the molecular mechanism of chickens’ taste sense defines the feeding behavior of chickens. Here we focused on the gene expressions in two different oral tissues of chickens – the palate, which contains many taste buds, and the tongue tip, which contains few taste buds. Using the quantitative real‐time polymerase chain reaction method, we found that the molecular markers for taste buds of chickens, that is α ‐gustducin and vimentin , were expressed significantly highly in the palate compared to the tongue tip. Our analyses also revealed that transient receptor potential subfamily M member 5 ( TRPM 5 ), a cation channel involved in taste transduction in mammals, was also highly expressed in the palate compared to the tongue tip. Our findings demonstrated that the expression patterns of these genes were significantly correlated. We showed that the aversion to bitter solution was alleviated by a TRPM 5 inhibitor in behavior of chickens. Taken together, our findings enabled us to develop a simple method for screening taste‐related genes in chickens. The use of this method demonstrated that TRPM 5 was involved in chickens’ taste transduction, and that a TRPM 5 inhibitor can alleviate chickens’ bitter taste perception of feed ingredients.