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Effects of ascorbic acid 2‐glucoside and alpha‐tocopherol on the characteristics of equine spermatozoa stored at 5°C
Author(s) -
Sampaio Breno Fernandes Barreto,
Nogueira Bruno Gomes,
Souza Maria Inês Lenz,
Silva Eliane Vianna da Costa e,
Zúccari Carmem Estefânia Serra Neto
Publication year - 2018
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12944
Subject(s) - lipid peroxidation , semen , ascorbic acid , chemistry , antioxidant , sperm , vitamin e , malondialdehyde , dna fragmentation , sperm motility , andrology , extender , biochemistry , fragmentation (computing) , vitamin c , biology , food science , medicine , apoptosis , ecology , programmed cell death , organic chemistry , polyurethane
The aim of this experiment was to evaluate the effects of adding ascorbic acid 2‐glucoside ( AA 2G), a water‐soluble antioxidant and stable derivative of ascorbate, to the semen extender and compare it to the addition of vitamin C (Vit. C) and the fat‐soluble antioxidant α‐tocopherol (α‐Toh), both individually and in combination, on the seminal variables of equine sperm submitted to cooling for 72 h. We used two ejaculates from 10 stallions and evaluated them for motility, membrane integrity, chromatin fragmentation, mitochondrial activity and lipid peroxidation. In the analysis of lipid peroxidation, the control group showed 2506.2 ± 796.4 ng malondialdehyde/10 8 sperm, which was higher ( P < 0.05) than the groups treated with antioxidants. The average value of motility in the AA 2G group was 68.4 ± 18.1%, which was higher ( P < 0.05) than that observed in the control group (62.1 ± 16.2%). The variables membrane integrity, chromatin fragmentation and mitochondrial activity did not show significant difference ( P > 0.05) between treatments. It was concluded that the antioxidants protected sperm cells from lipid peroxidation and that AA 2G was effective during the cooling process of equine semen at 5°C for72 h, providing increased levels of total motility.