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Trans10, cis12 conjugated linoleic acid increases triacylglycerol accumulation in goat mammary epithelial cells in vitro
Author(s) -
Zhang Tianying,
Ma Yue,
Wang Hui,
Loor Juan J,
Xu Huifen,
Shi Huaiping,
Luo Jun
Publication year - 2018
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12935
Subject(s) - conjugated linoleic acid , in vitro , linoleic acid , chemistry , mammary gland , biochemistry , food science , biology , microbiology and biotechnology , fatty acid , cancer , genetics , breast cancer
Trans10, cis12 conjugated linoleic acid (t10c12‐ CLA ) is well‐established in decreasing milk fat content and causing milk fat depression ( MFD ) in dairy cattle and goats. However, the detailed mechanisms of its effect are not completely understood. Therefore, we used goat mammary epithelial cells ( GMEC s) to further study the molecular mechanisms whereby t10c12‐ CLA regulates milk fat synthesis. The optimal concentration of t10c12‐ CLA (100 μmol/L) for cell culture was determined through a cell vitality and morphology assay, and evaluation of abundance of apoptosis‐related proteins. Oil red O stain indicated that t10c12‐ CLA increased concentration of cytoplasmic lipid droplets. Furthermore, t10c12‐ CLA increased the intracellular triacylglycerol ( TG ) content ( P < 0.05). Among 16 genes related to lipid metabolism that were measured by quantitative real‐time PCR , t10c12‐ CLA down‐regulated ( P < 0.05) genes involved in de novo fatty acid synthesis including FASN , ACACA and SCD 1 , and also down‐regulated the protein expression of FASN and SCD 1 but up‐regulated ( P < 0.05) the expression of CD 36 and ADRP . Overall, the data indicate that a side effect of de novo fatty acid synthesis inhibition by t10c12‐ CLA is the up‐regulation of fatty acid uptake and accumulation of lipid droplets in GMEC s. The biologic reason for such an effect merits further study.