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Partial characterization of phylogeny, ecology and function of the fibrolytic bacterium Ruminococcus flavefaciens OS 14, newly isolated from the rumen of swamp buffalo
Author(s) -
Boonsaen Phoompong,
Kinjo Madoka,
Sawa Suriya,
Suzuki Yutaka,
Koike Satoshi,
Kobayashi Yasuo
Publication year - 2018
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12927
Subject(s) - biology , ruminococcus , rumen , bacteria , 16s ribosomal rna , phylogenetic tree , swamp , phylogenetics , microbiology and biotechnology , strain (injury) , food science , feces , fermentation , biochemistry , ecology , gene , genetics , anatomy
The fibrolytic rumen bacterium Ruminococcus flavefaciens OS 14 was isolated from swamp buffalo and its phylogenetic, ecological and digestive properties were partially characterized. Isolates from rumen contents of four swamp buffalo were screened for fibrolytic bacteria; one of the 40 isolates showed a distinctive feature of solubilizing cellulose powder in liquid culture and was identified as R. flavefaciens based on its 16S ribosomal DNA sequence. This isolate, OS 14, was employed for detection and digestion studies, for which a quantitative PCR assay was developed and defined cultures were tested with representative forages in Thailand. OS 14 was phylogenetically distant from other isolated and uncultured R. flavefaciens and showed limited distribution among Thai ruminants but was absent in Japanese cattle. OS 14 digested rice straw and other tropical forage to a greater extent than the type strain C94 of R. flavefaciens . OS 14 produced more lactate than C94, and digested para grass to produce propionate more extensively in co‐culture with lactate‐utilizing Selenomonas ruminantium S137 than a co‐culture of C94 with S137. These results indicate that phylogenetically distinct OS 14 could digest Thai local forage more efficiently than the type strain, possibly forming a symbiotic cross‐feeding relationship with lactate‐utilizing bacteria. This strain might be useful for future animal and other industrial applications.