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Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts
Author(s) -
Takaya Tomohide,
Nihashi Yuma,
Kojima Shotaro,
Ono Tamao,
Kagami Hiroshi
Publication year - 2017
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12884
Subject(s) - myogenesis , green fluorescent protein , myocyte , microbiology and biotechnology , multinucleate , cell fusion , c2c12 , biology , fusion protein , skeletal muscle , cell , gene , biochemistry , anatomy , recombinant dna
Cell‐cell fusion has been a great technology to generate valuable hybrid cells and organisms such as hybridomas. In this study, skeletal muscle myoblasts were utilized to establish a novel method for autonomous xenogenic cell fusion. Myoblasts are mononuclear myogenic precursor cells and fuse mutually to form multinuclear myotubes. We generated murine myoblasts ( mMB s) expressing green fluorescent protein ( GFP ) termed mMB ‐ GFP , and the chick myoblasts (ch MB s) expressing Discosoma red fluorescent protein (DsRed) termed ch MB ‐DsRed. mMB ‐ GFP and ch MB ‐DsRed were cocultured and induced to differentiate. After 24 h, the multinuclear myotubes expressing both GFP and DsRed were observed, indicating that mMB s and ch MB s interspecifically fuse. These GFP + /DsRed + hybrid myotubes were able to survive and grew to hyper‐multinucleated mature form. We also found that undifferentiated mMB ‐ GFP efficiently fuse to the ch MB ‐DsRed‐derived myotubes. This is the first evidence for the autonomous xenogenic fusion of mammalian and avian cells. Myoblast‐based fusogenic technique will open up an alternative direction to create novel hybrid products.