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The quality after culture in vitro or in vivo of porcine oocytes matured and fertilized in vitro and their ability to develop to term
Author(s) -
Nakamura Yoshiyuki,
Tajima Sigeyuki,
Kikuchi Kazuhiro
Publication year - 2017
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12855
Subject(s) - blastocyst , andrology , in vivo , embryo , in vitro , embryo culture , oocyte , in vitro maturation , biology , in vitro fertilisation , cryopreservation , embryogenesis , human fertilization , chemistry , anatomy , medicine , microbiology and biotechnology , genetics
The quality of porcine blastocysts produced in vitro is poor in comparison with those that develop in vivo . We examined the quality of in vitro ‐matured and fertilized ( IVM / IVF ) oocytes, their abilities to develop to blastocysts under in vivo and in vitro conditions, and the potential of the embryos to develop to term after transfer. IVM / IVF oocytes were either transferred and the embryos recovered on Days 5 and 6 (100% and 87.5%, respectively) (‘ ET ‐vivo’ embryos), or cultured in vitro for 5 or 6 days (‘ IVC ’ embryos). The proportion of blastocysts differed significantly between the two groups on Day 5 (20.6% and 8.0%, respectively), but not on Day 6 (23.8% and 21.2%, respectively). The mean number of cells in ET ‐vivo blastocysts on Days 5 or 6 was significantly higher (72.8 and 78.7, respectively) than that in IVC blastocysts (22.1 and 39.7, respectively). When IVM / IVF oocytes and IVC blastocysts on Day 6 were transferred, all (three and three, respectively) developed to piglets (16 and 16, respectively), without any difference in the rates of development to term (2.1% and 2.6%, respectively). These data suggest that, although blastocyst production differs between the two culture conditions, IVM / IVF oocytes possess the same ability to develop to term.