Premium
Extension of the culture period for the in vitro growth of bovine oocytes in the presence of bone morphogenetic protein‐4 increases oocyte diameter, but impairs subsequent developmental competence
Author(s) -
Yang Yinghua,
Kanno Chihiro,
Sakaguchi Kenichiro,
Yanagawa Yojiro,
Katagiri Seiji,
Nagano Masashi
Publication year - 2017
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12841
Subject(s) - oocyte , blastocyst , in vitro maturation , andrology , human fertilization , in vitro , biology , bone morphogenetic protein , embryo , bone morphogenetic protein 15 , endocrinology , embryogenesis , medicine , microbiology and biotechnology , anatomy , bone morphogenetic protein 7 , genetics , gene
Bone morphogenetic protein‐4 ( BMP ‐4) inhibits luteinization of granulosa cells during in vitro growth ( IVG ) culture of bovine oocytes; however, oocytes derived from a 12 day IVG were less competent for development than in vivo ‐grown oocytes. We herein investigated whether an extended IVG culture with BMP ‐4 improves oocyte growth and development to blastocysts after in vitro fertilization. Oocyte‐granulosa cell complexes ( OGC s) were cultured for 14 or 16 days with BMP ‐4 (10 ng/mL), while a 12 day culture with BMP ‐4 served as the in vitro control. OGC viability was maintained for the 16 day culture with BMP ‐4 (83.2%), but was significantly lower without BMP ‐4 (58.9%) than the control (83.0%). Prolong‐cultured oocytes at 16 days had statistically greater diameter (114.6 μm) than the control (111.7 μm). IVG oocytes with BMP ‐4 for the 16 day culture had a similar nuclear maturation rate to the control (approximately 67%); however, blastocyst rates in BMP ‐4 treated oocytes of 14 (1.8%) and 16 day (0%) IVG were statistically lower than that of 12 day IVG (9.0%). In conclusion, BMP ‐4 maintained OGC viability and promoted oocyte growth in a prolonged culture, but impaired the developmental competence of oocytes. Prolonged culture may not be an appropriate strategy for enhancing the developmental competence of IVG oocytes.