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Effects of supplementation of iodixanol to semen extender on quality and fertilization ability of frozen–thawed Thai native bull sperm
Author(s) -
Chuawongboon Phirawit,
Sirisathien Saksiri,
Pongpeng Jatuporn,
Sakhong Denpong,
Nagai Takashi,
Vongpralub Thevin
Publication year - 2017
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12798
Subject(s) - extender , iodixanol , andrology , sperm , human fertilization , semen extender , semen , acrosome , cryopreservation , semen quality , membrane integrity , in vitro fertilisation , biology , artificial insemination , sperm motility , insemination , zoology , chemistry , anatomy , embryo , pregnancy , medicine , biochemistry , genetics , organic chemistry , membrane , contrast medium , polyurethane , radiology , microbiology and biotechnology
Abstract This study investigates the effects of iodixanol supplementation in varied concentrations to Tris egg yolk ( TEY ) extender on the quality and fertilization ability of frozen–thawed sperm of Thai native bulls. Each ejaculate was divided into four different groups, as follows: sperm were treated with TEY extender (control group) and TEY extender supplemented with three different concentrations of iodixanol (1.25%, 2.50% and 5.00%). Semen straws were frozen in liquid nitrogen vapor. After thawing, sperm motility characteristics, viability, plasma membrane integrity and acrosome integrity were determined. Also, frozen–thawed spermatozoa from all groups were used for in vitro fertilization and artificial insemination ( AI) in natural estrus Thai native cows. The results showed that the post‐thaw quality of the 2.50% iodixanol group was superior to the other iodixanol groups ( P < 0.05). However, iodixanol had no beneficial effect on post‐thaw sperm in vitro fertilization ability and pregnancy rate after AI ( P > 0.05). It can be concluded that the supplementation of 2.50% iodixanol extender significantly improves the progressive motility, viability, plasma membrane integrity and acrosome integrity of cryopreserved semen from Thai native bulls, but it has no beneficial effect on in vitro fertilization ability and pregnancy rate after AI .