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Effect of supplemented sericin on the development, cell number, cryosurvival and number of lipid droplets in cultured bovine embryos
Author(s) -
Hosoe Misa,
Inaba Yasushi,
Hashiyada Yutaka,
Imai Kei,
Kajitani Kenji,
Hasegawa Yuichi,
Irie Mamoru,
Teramoto Hidetoshi,
Takahashi Toru,
Niimura Sueo
Publication year - 2017
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12628
Subject(s) - sericin , blastula , embryo , andrology , blastocyst , fetal bovine serum , chemistry , in vivo , in vitro , embryogenesis , biology , biochemistry , microbiology and biotechnology , materials science , silk , medicine , gastrulation , composite material
Abstract Sericin was investigated as an alternative to fetal bovine serum (FBS) for bovine embryo culture. In vitro matured oocytes were developed using 0.05%, 0.1% or 0.15% sericin. The developmental rate, cryosurvival rate and blastulation time of these embryos were compared with those of embryos developed using 5% FBS. The number of lipid droplets was compared among the blastocysts developed using 5% FBS, using 0.05% sericin and in vivo . The rate of cleavage and blastocyst formation was similar among all groups. Blastulation occurred significantly earlier in the embryos developed using 5% FBS than in those developed using sericin at any concentration ( P < 0.05). At 72 h after thawing, the cryosurvival rate of the blastocysts developed using 5% FBS and 0.05% sericin were significantly higher compared with those developed using 0.1% and 0.15% sericin ( P < 0.05). The blastocysts developed using 0.05% sericin and in vivo produced a significantly fewer number of medium and large lipid droplets than those developed using 5% FBS. These results suggest that the blastocysts developed using 0.05% sericin show characteristics similar to those of the blastocysts developed in vivo and that the use of sericin as an alternative to FBS is feasible.