Premium
Expression of DNA repair genes in porcine oocytes before and after fertilization by ICSI using freeze‐dried sperm
Author(s) -
Men Nguyen Thi,
Kikuchi Kazuhiro,
Furusawa Tadashi,
DangNguyen Thanh Quang,
Nakai Michiko,
Fukuda Atsunori,
Noguchi Junko,
Kaneko Hiroyuki,
Viet Linh Nguyen,
Xuan Nguyen Bui,
Tajima Atsushi
Publication year - 2016
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12554
Subject(s) - biology , sperm , gene , rad51 , human fertilization , dna fragmentation , msh2 , gene expression , dna repair , andrology , dna , microbiology and biotechnology , genetics , dna mismatch repair , apoptosis , medicine , programmed cell death
Boar sperm freeze‐dried with trehalose showed a protective effect against sperm DNA fragmentation. However, normal fertilization and embryonic development were not improved. Damaged sperm may activate maternal DNA repair genes when injected into oocytes. Therefore, we investigated the expression profile of some DNA repair genes in porcine oocytes after intra‐cytoplasmic sperm injection. First, the expression levels of MGMT , UDG , XPC , MSH2 , XRCC6 and RAD51 genes that are concerned with different types of DNA repair were examined in in vitro mature (IVM) oocytes injected with ejaculated sperm, or freeze‐dried sperm with or without trehalose. Quantitative reverse transcription polymerase chain reaction revealed that expression of six DNA repair genes in the oocytes at 4 h after injection did not differ among the four groups. Next, we investigated the gene expression levels of these genes at different stages of maturation. The relative expression levels of UDG and XPC were significantly up‐regulated in mature oocytes compared with earlier stages. Furthermore, there was an increased tendency in relative expression of MSH2 and RAD51 . These results suggested two possible mechanisms that messenger RNA of DNA repair genes are either accumulated during IVM to be ready for fertilization or increased expression levels of DNA repair genes in oocytes caused by suboptimal IVM conditions.