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The suppressive effect of dietary coenzyme Q 10 on mitochondrial reactive oxygen species production and oxidative stress in chickens exposed to heat stress
Author(s) -
Kikusato Motoi,
Nakamura Kasumi,
Mikami Yukiko,
Mujahid Ahmad,
Toyomizu Masaaki
Publication year - 2016
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12543
Subject(s) - coenzyme q10 , reactive oxygen species , oxidative stress , mitochondrial ros , skeletal muscle , mitochondrion , antioxidant , oxidative phosphorylation , coenzyme q – cytochrome c reductase , biochemistry , biology , inner mitochondrial membrane , medicine , chemistry , endocrinology , cytochrome c
This study was conducted to determine if dietary supplementation with coenzyme Q 10 (CoQ 10 ), which can act as a potent antioxidant and is an obligatory cofactor of mitochondrial uncoupling protein, suppresses the heat stress (HS)‐induced overproduction of mitochondrial reactive oxygen species (ROS) and oxidative damage in the skeletal muscle of birds. The carbonyl protein content of skeletal muscle was significantly higher in birds exposed to HS treatment (34°C, 12 h) than in thermoneutral birds (25°C). This increase was suppressed by CoQ 10 supplementation (40 mg/kg diet). Succinate‐supported mitochondrial ROS production was increased by HS treatment, and this increase was also suppressed by CoQ 10 supplementation. In contrast, CoQ 10 supplementation did not affect the HS‐induced decrease in mitochondrial proton leak. The mitochondrial membrane potential (ΔΨ), to which HS‐induced ROS production was previously shown to be sensitive, tended to be increased by HS treatment, but this rise in ΔΨ was not affected by CoQ 10 supplementation. Taken together, these results suggest that dietary CoQ 10 supplementation attenuates HS‐induced oxidative damage to skeletal muscle, by preventing the overproduction of succinate‐supported mitochondrial ROS in a manner that is independent of ΔΨ. © 2015 Japanese Society of Animal Science