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Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development
Author(s) -
Egashira Akiyoshi,
Yamauchi Nobuhiko,
Islam Md. Rashedul,
Yamagami Kazuki,
Tanaka Asami,
Suyama Hikaru,
ElSayed ElSharawy Mohamed,
Tabata Shoji,
Kuramoto Takashi
Publication year - 2016
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12530
Subject(s) - blastocyst , embryo , andrology , gene knockdown , blastomere , germinal vesicle , multinucleate , biology , oocyte , microbiology and biotechnology , messenger rna , embryogenesis , zygote , apoptosis , gene , genetics , medicine
Abstract This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1‐ and 2‐cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher ( P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4‐cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2‐ to 4‐cell arrested embryos and reduce the developmental competence to blastocyst.