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Effective use of the TSPY gene‐specific copy number in determining fetal DNA in the maternal blood of cynomolgus monkeys
Author(s) -
Yasmin Lubna,
Takano Junichiro,
Sankai Tadashi
Publication year - 2016
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12523
Subject(s) - testis determining factor , taqman , fetus , dna , biology , real time polymerase chain reaction , cell free fetal dna , microbiology and biotechnology , gene , polymerase chain reaction , andrology , genomic dna , pregnancy , genetics , prenatal diagnosis , y chromosome , medicine
Since the available concentration of single‐copy fetal genes in maternal blood DNA is sometimes lower than detection limits by PCR methods, the development of specific and quantitative PCR detection methods for fetal DNA in maternal blood is anticipated, which may broaden the methods that can be used to monitor pregnancy. We used the TaqMan qPCR amplification for DYS14 multi‐copy sequence and the SRY gene in maternal blood plasma (cell‐free DNA) and fractional precipitated blood cells (cellular DNA) from individual cynomolgus monkeys at 22 weeks of pregnancy. The availability of cell‐free fetal DNA was higher in maternal blood plasma than that of cellular DNA from fractional precipitated blood cells. There was a significantly higher ( P < 0.001) mean copy number of fetal male DYS14 from maternal plasma (4.4 × 10 4 copies/mL) than that of detected fetal cellular DNA from fractional blood cell pellets. The sensitivity of the DYS14 PCR assay was found to be higher than that of the SRY assay for the detection of fetal DNA when its presence was at a minimum. The DYS14 assay is an improved method for quantifying male fetal DNA in circulating maternal blood in the primate model.