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Utilization of digital differential display to identify differentially expressed genes related to rumen development
Author(s) -
Kato Daichi,
Suzuki Yutaka,
Haga Satoshi,
So KyoungHa,
Yamauchi Eri,
Nakano Miwa,
Ishizaki Hiroshi,
Choi Kichoon,
Katoh Kazuo,
Roh SangGun
Publication year - 2016
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12448
Subject(s) - rumen , omasum , biology , gene , reticulum , abomasum , candidate gene , gene expression , in silico , andrology , genetics , biochemistry , medicine , endoplasmic reticulum , fermentation
Abstract This study aimed to identify the genes associated with the development of the rumen epithelium by screening for candidate genes by digital differential display (DDD) in silico . Using DDD in NCBI's UniGene database, expressed sequence tag (EST)‐based gene expression profiles were analyzed in rumen, reticulum, omasum, abomasum and other tissues in cattle. One hundred and ten candidate genes with high expression in the rumen were derived from a library of all tissues. The expression levels of 11 genes in all candidate genes were analyzed in the rumen, reticulum, omasum and abomasum of nine Japanese Black male calves (5‐week‐old pre‐weaning: n = 3; 15‐week‐old weaned calves: n = 6). Among the 11 genes, only 3‐hydroxy‐3‐methylglutaryl‐CoA synthase 2 ( HMGCS2 ), aldo‐keto reductase family 1, member C1‐like ( AKR1C1 ), and fatty acid binding protein 3 ( FABP3 ) showed significant changes in the levels of gene expression in the rumen between the pre‐ and post‐weaning of calves. These results indicate that DDD analysis in silico can be useful for screening candidate genes related to rumen development, and that the changes in expression levels of three genes in the rumen may have been caused by weaning, aging or both. © 2015 Japanese Society of Animal Science