Premium
Basic characterization of avian β‐defensin genes in the Japanese quail, Coturnix japonica
Author(s) -
Ishige Taichiro,
Hara Hiromi,
Hirano Takashi,
Mannen Hideyuki,
Kono Tomohiro,
Hanzawa Kei
Publication year - 2016
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12436
Subject(s) - biology , synteny , genetics , quail , locus (genetics) , gene , coturnix japonica , coturnix , coturnix coturnix , phylogenetic tree , gene cluster , sequence analysis , gene duplication , chromosome , endocrinology
In this study, we identified a cluster of 14 avian β‐defensins ( AvBD ; approximately 66 kbp) in the Japanese quail, Coturnix japonica . Except for AvBD12 ( CjAvBD12 ) and ‐ 13 , the CjAvBDs coding sequences exhibited greater than 78.0% similarity to the respective orthologous chicken AvBD genes ( GgAvBD ). The putative amino acid sequence encoded by each CjAvBD contained six cysteine residues and the GXC (X1‐2) motif considered essential for the β ‐defensin family. Each CjAvBDs also formed a sub‐group with the respective orthologous genes of various bird species in a phylogenetic tree analysis. Synteny between the CjAvBD cluster and GgAvBD cluster was confirmed. The CjAvBD cluster was mapped on the long‐arm end of chromosome 3 by linkage analysis based on single nucleotide polymorphisms (SNPs) of CjAvBD1 and CjAvBD12 (approximately 46kbp), as well as GgAvBD cluster. We also confirmed that CjAvBD1 , ‐ 4 , ‐ 5 , ‐ 9 , and ‐ 10 are transcribed in 20 tissues, including immune and digestive tissues. However, our experimental data indicated that the CjAvBD cluster lacks the AvBD3 and ‐ 7 loci, whereas the CjAvBD101α , ‐ 101β , and ‐ 101θ loci arose from gene duplication of the AvBD6 orthologous locus in the CjAvBD cluster after differentiation between Coturnix ‐ Gallus .