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The effect of C urcuma longa extracted (curcumin) on the quality of cryopreserved boar semen
Author(s) -
Chanapiwat Panida,
Kaeoket Kampon
Publication year - 2015
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12395
Subject(s) - extender , semen , cryopreservation , curcumin , andrology , semen cryopreservation , acrosome , boar , semen extender , chemistry , artificial insemination , sperm motility , semen quality , insemination , zoology , sperm , biology , biochemistry , medicine , embryo , pregnancy , genetics , organic chemistry , polyurethane , microbiology and biotechnology
The aim of this study was to determine the optimal concentration of curcumin needed for cryopreservation of boar semen. Semen samples ( n = 9) were collected from nine D uroc boars which having proven fertility were used for routine artificial insemination. Semen samples were collected and divided into six groups (groups A‐F) according to various concentrations of curcumin in freezing extender (i.e. 0, 0.125, 0.25, 0.50, 0.75 and 1.0 mmol/L, respectively). The semen was frozen by traditional liquid nitrogen vapor method and stored at −196°C in the liquid nitrogen tank. After storage, frozen semen samples were thawed at 50°C for 12 s and evaluated for progressive motility, viability and acrosome integrity. The present results indicated that the addition of curcumin at 0.25 (group C) or 0.50 mmol/L curcumin (group D) yielded the higher percentage of progressive motility (33.3 and 36.1%, respectively) ( P < 0.001). A significantly higher percentage of acrosome integrity was found in groups B (29.7%), C (31.1%) and D (30.2%) than in the other groups ( P < 0.01). However, there was no significant difference in percentage of viability among groups. In conclusion, addition to the freezing extender of curcumin during cryopreservation at a concentration of 0.25 or 0.50 mmol/L is the optimal concentration of curcumin for improving the quality (i.e. increased progressive motility and acrosome integrity) of cryopreserved boar semen.