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Early post mortem expression of genes related to tenderization in two I talian S immental young bulls' skeletal muscles differing in contractile type
Author(s) -
Saccà Elena,
Corazzin Mirco,
Pizzutti Nicoletta,
Lippe Giovanna,
Piasentier Edi
Publication year - 2015
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12386
Subject(s) - calpastatin , myosin , calpain , ageing , tenderness , messenger rna , hsp70 , gene isoform , chemistry , heat shock protein , meat tenderness , skeletal muscle , hsp27 , gene expression , andrology , biology , microbiology and biotechnology , endocrinology , medicine , biochemistry , gene , food science , enzyme , genetics
The early post mortem expression of eight genes potentially involved in meat ageing process and the tenderness of two Italian Simmental young bulls' ( B os taurus ) skeletal muscles differing in their contractile type were evaluated. Samples of L ongissimus lumborum ( LL ) and I nfraspinatus ( IS ) muscles were collected from 17 bulls. The messenger RNA ( mRNA ) abundances of calpain‐1, calpain‐2, calpastatin, caspase 3, caspase 9, heat shock p r otein 27 (Hsp27), H sp40 and H sp70 were detected by quantitative PCR . The myosin heavy chain‐slow and ‐fast isoform content, the pH 48h and the lipid content of the muscles were in line with the contractile and metabolic type. In comparison with the fast LL , the slow IS showed a lower calpain‐1/calpastatin mRNA content ratio after slaughtering and a higher W arner‐ B ratzler I nitial Y ield value after 7 days of ageing. H sp27 and H sp70 mRNA abundances were significantly lower in LL than IS , highlighting their potential role in the ageing process of bovine muscles.