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Grazing‐induced changes in muscle microRNA ‐206 and ‐208b expression in association with myogenic gene expression in cattle
Author(s) -
Horikawa Akihiko,
Ogasawara Hideki,
Okada Kaito,
Kobayashi Misato,
Muroya Susumu,
Hojito Masayuki
Publication year - 2015
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12381
Subject(s) - grazing , myod , biology , microrna , myogenin , beef cattle , gene expression , skeletal muscle , gene , endocrinology , zoology , genetics , agronomy
Abstract To investigate the roles of microRNAs ( miRNAs ) in muscle type conversion, the effects of 4 months of grazing on the expression levels of miRNAs and mRNAs associated with skeletal muscle development were analyzed by quantitative RT‐PCR using the Biceps femoris muscle of J apanese Shorthorn cattle. After 4 months of grazing, the expression of muscle fiber type‐associated mi R ‐208b was higher in the grazed cattle than in the housed. In concordance with the pattern in mi R ‐208b expression, the expression of MyoD , a myogenic regulatory factor associated with the shifting of muscle property to the fast type, was lower in the grazed cattle after 4 months of grazing than in the housed cattle. In addition, the expression of MyHC ‐2x (a fast type) was higher in the housed cattle than in the grazed, after 4 months of grazing. During the grazing period, mi R ‐206 expression decreased in the housed cattle, whereas expression in the grazed cattle did not change, but rather remained higher than that of the housed cattle even at 3 months after the grazing ended. These miRNAs including mi R ‐206 persisting with muscles of grazed cattle may be associated with regulation of muscle gene expression during skeletal muscle adaptation to grazing.