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Effects of different roughage sources and feeding levels on adipogenesis of ovine adipocytes
Author(s) -
Kim DaHye,
Choi KiChoon,
Ichinohe Toshiyoshi,
Song SangHoun
Publication year - 2015
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12380
Subject(s) - adipogenesis , adipocyte , hay , adipose tissue , medicine , ruminant , endocrinology , biology , zoology , chemistry , agronomy , crop
The objective of the present study was to conduct an adipogenic evaluation of different roughage sources and feeding levels during ruminant adipocyte differentiation in vitro . Six wether sheep were divided into a timothy hay feeding group ( TFG , n = 3) and an I talian ryegrass straw feeding group ( IFG , n = 3). The sheep were fed high‐roughage ( HR ), medium roughage ( MR ) and low‐roughage ( LR ) diets in a one‐way layout design each over a 6‐day period. Sheep serum samples collected on the last day of each dietary treatment were added to an adipogenic induction medium for differentiation of preadipocytes derived from sheep subcutaneous adipose tissue. The cytoplasmic lipid accumulations in the TFG serum‐treated preadipocytes were significantly higher than those of the IFG ‐serum treated preadipocytes on day 12. Messenger RNA expression of CCAAT/enhancer‐binding protein ( C / EBP )‐α, C / EBP ‐β, C / EBP ‐δ, fatty‐acid‐binding protein (a P 2) and stearoyl‐coenzyme A desaturase ( SCD ) were regulated by each serum treatment. This study shows that different roughage source diets and roughage‐to‐concentrate ratio diets can regulate adipocyte differentiation via ruminant blood composition.