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Sirt1 protects pig oocyte against in vitro aging
Author(s) -
Ma Rujun,
Zhang Yu,
Zhang Liang,
Han Jun,
Rui Rong
Publication year - 2015
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12360
Subject(s) - oocyte , resveratrol , in vitro , biology , activator (genetics) , microbiology and biotechnology , sirtuin 1 , andrology , in vitro maturation , mitochondrion , ageing , genetics , embryo , gene , medicine , downregulation and upregulation , biochemistry
Sirtuins have been widely reported to be involved in multiple biological processes. However, their function during pig oocyte aging has not been reported yet. Here, we first identify that sirt1 expression is dramatically reduced in pig in vitro ‐aged oocytes. Furthermore, by confocal scanning and quantitative analysis, we find the increased frequency of spindle defects and chromosome misalignment, disturbed redistribution of cortical granules and mitochondria during oocyte in vitro ‐aging. Importantly, these aging‐associated defective phenotypes can be ameliorated through resveratrol (sirt1 activator) treatment during pig oocyte maturation, providing the evidence for the hypothesis that decreased sirt1 is one of a number of factors contributing to oocyte in vitro ‐aging. In summary, our data indicate a role for sirt1 in pig oocytes and uncover a striking beneficial effect of sirt1 expression on aged oocytes.