Premium
The g.841G> C SNP of FASN gene is associated with fatty acid composition in beef cattle
Author(s) -
Hayakawa Kiri,
Sakamoto Takayuki,
Ishii Atsushi,
Yamaji Keita,
Uemoto Yoshinobu,
Sasago Nanae,
Kobayashi Eiji,
Kobayashi Naohiko,
Matsuhashi Tamako,
Maruyama Shin,
Matsumoto Hirokazu,
Oyama Kenji,
Mannen Hideyuki,
Sasazaki Shinji
Publication year - 2015
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12357
Subject(s) - single nucleotide polymorphism , biology , fatty acid , population , gene , genotyping , fatty acid synthase , genetics , snp , genotype , biochemistry , medicine , environmental health
The objective of the current study is to evaluate the association between fatty acid composition and fatty acid synthase gene polymorphisms as responsible mutations. For this purpose, we selected seven previously reported single nucleotide polymorphisms ( SNPs ) in FASN gene, including one within promoter region (g.841 G > C ) and six non‐synonymous SNPs (g.8805 C > T , g.13126 C > T , g.15532 A > C , g.16024 A > G , g.16039 C > T , g.17924 A > G ), and genotyped them in J apanese B lack cattle. Genotyping results revealed that g.8805 C > T and g.17924 A > G were monomorphic loci. Genome‐wide association analysis including the other five SNPs revealed that only g.841 G > C showed significant associations with the percentages of C 14:0, C 14:1, C 16:1 and C 18:1 at 5% genome‐wide significance level. In order to further evaluate the effect, we genotyped g.841 G > C using additional three populations, including two J apanese B lack populations and a H olstein cattle population. g.16024 A > G was also genotyped and included in the analysis because it has been reported to be associated with fatty acid composition in J apanese B lack cattle. In the result of analysis of variance, g.841 G > C showed stronger effects on fatty acid percentage than those of g.16024 A > G in all populations. These results suggested that g.841 G > C would be a responsible mutation for fatty acid composition and contribute to production of high‐grade beef as a selection marker in beef cattle.