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Conjugated fatty acids and methane production by rumen microbes when incubated with linseed oil alone or mixed with fish oil and/or malate
Author(s) -
Li Xiang Z.,
Gao Qing S.,
Yan Chang G.,
Choi Seong H.,
Shin Jong S.,
Song Man K.
Publication year - 2015
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12354
Subject(s) - linseed oil , rumen , fish oil , propionate , food science , conjugated linoleic acid , chemistry , linolenic acid , incubation , linoleic acid , methanogen , fatty acid , biochemistry , biology , zoology , fermentation , methane , fish <actinopterygii> , organic chemistry , fishery
We hypothesized that manipulating metabolism with fish oil and malate as a hydrogen acceptor would affect the biohydrogenation process of α‐linolenic acid by rumen microbes. This study was to examine the effect of fish oil and/or malate on the production of conjugated fatty acids and methane ( CH 4 ) by rumen microbes when incubated with linseed oil. Linseed oil ( LO ), LO with fish oil ( LO‐FO ), LO with malate ( LO‐MA ), or LO with fish oil and malate ( LO‐FO ‐ MA ) was added to diluted rumen fluid, respectively. The LO‐MA and LO‐FO ‐ MA increased pH and propionate concentration compared to the other treatments. LO‐MA and LO‐FO ‐ MA reduced CH 4 production compared to LO . LO‐MA and LO‐FO ‐ MA increased the contents of c9,t11‐conjugated linoleic acid ( CLA ) and c9,t11,c15‐conjugated linolenic acid ( CLnA ) compared to LO . The content of malate was rapidly reduced while that of lactate was reduced in LO‐MA and LO‐FO ‐ MA from 3 h incubation time. The fold change of the quantity of methanogen related to total bacteria was decreased at both 3 h and 6 h incubation times in all treatments compared to the control. Overall data indicate that supplementation of combined malate and/or fish oil when incubated with linseed oil, could depress methane generation and increase production of propionate, CLA and CLnA under the conditions of the current in vitro study.