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Stimulatory effect of plasma samples from fattening cattle on adipogenesis‐related gene expression in preadipocyte cells
Author(s) -
Watanabe Hitoshi,
Chen Xiangning,
Shoji Noriaki,
Saito Ryo,
Nakano Tatsuya,
Saito Kazuki,
Sumiyoshi Keisuke,
Rose Michael T.,
Okada Natumi,
Watanabe Kouichi,
Aso Hisashi
Publication year - 2015
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12344
Subject(s) - nefa , intramuscular fat , adipogenesis , medicine , endocrinology , messenger rna , adipocyte , fatty acid , gene expression , biology , population , chemistry , peroxisome , biochemistry , adipose tissue , gene , demography , sociology
It is desirable to produce beef with high levels of monounsaturated fatty acids ( MUFA ), as this is related to fat softness and palatability. However, the physiology of MUFA synthesis in bovine fat during the fattening process remains to be established. In this study, in order to elucidate the relationship between plasma components and the fatty acid composition of intramuscular fat, we investigated the effect of plasma obtained from fattening cattle on the messenger RNA ( mRNA ) expressions of the adipogenesis‐related gene in a clonal bovine intramuscular preadipocyte line ( BIP cells). The mRNA expressions of stearoyl‐ CoA desaturase, adipocyte P rotein 2, peroxisome proliferator‐activated receptor gamma and sterol regulatory element‐binding protein 1 in BIP cells were significantly higher following treatment with those plasma samples collected from the cattle with the highest diaphragmatic unsaturated fatty acids to saturated fatty acids ratio ( US / S ). Furthermore, the concentration of nonesterified fatty acid ( NEFA ) in the plasma samples had an inverse correlation with carcass diaphragmatic US / S . These results indicate that cattle with a low ratio of US / S in fat may be discriminated from the population of fattening cattle before slaughter by measuring the effect of their plasma on gene expression in BIP cells as well as their plasma concentration and composition of NEFA .