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Protective effect of aqueous extracts from R hizopus oryzae on liver injury induced by carbon tetrachloride in rats
Author(s) -
Suzuki Takehito,
Fukuoka Hideo,
Ushikoshi Setsuo,
Sato Reiichiro,
Morita Hidetoshi,
Takizawa Tatsuya
Publication year - 2015
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12328
Subject(s) - carbon tetrachloride , ccl4 , chemistry , liver injury , h&e stain , lactate dehydrogenase , oral administration , hepatocyte , hepatocyte growth factor , endocrinology , pharmacology , toxicity , medicine , biochemistry , immunohistochemistry , enzyme , in vitro , receptor , organic chemistry
Hepatoprotective effects of R hizopus oryzae/ U ‐1 aqueous extract ( RU ) were demonstrated in carbon tetrachloride ( CCl 4 )‐induced liver‐injured rats. In order to investigate the RU effects, the rats were administered RU at a dose of 10 or 100 mg/kg of body weight for 10 days before induction of the liver injury by oral administration of CCl 4 (125 mg/kg body weight). (i) Pretreatment with RU caused a significant decrease in serum lactate dehydrogenase ( LDH ), alanine aminotransferase ( ALT ) and aspartate aminotransferase ( AST ) activities that were increased by the administration of CCl 4 . (ii) RU pretreatment (100 mg/kg) increased 5‐bromo‐2′‐deoxyuridine incorporation at 48 h after CCl 4 treatment in hepatocytes. (iii) Histological hematoxylin and eosin staining of the liver showed that RU pretreatment reduced the damage induced by CCl 4 administration. (iv) Reverse transcriptase PCR analysis showed RU retreatment caused a transient but significant increase in hepatocyte growth factor ( HGF ) and a sustained and significant increase in insulin‐like growth factor‐ I ( IGF ‐ I ) gene expression in hepatocytes injured by CCl 4 treatment. From these results, we conclude that oral pre‐administration of RU was effective to suppress liver injury induced by the subsequent oral CCl 4 administration, and RU ‐induced increase in IGF ‐ I and HGF gene expression may be, even in part, involved in biological actions of RU in rats.