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Production of B an miniature pig embryos by in vitro fertilization: A comparative study with L andrace
Author(s) -
Nguyen Bui Xuan,
Kikuchi Kazuhiro,
Uoc Nguyen Thi,
DangNguyen Thanh Quang,
Linh Nguyen Viet,
Men Nguyen Thi,
Nguyen Trung Thanh,
Nagai Takashi
Publication year - 2015
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12317
Subject(s) - breed , andrology , embryo , equine chorionic gonadotropin , biology , human fertilization , in vitro fertilisation , sperm , human chorionic gonadotropin , in vitro maturation , oocyte , hormone , endocrinology , zoology , ovulation , anatomy , genetics , medicine
Abstract B an is an endangered miniature pig breed in V ietnam. This study aimed to set up an in vitro embryo production ( IVP ) system for this breed. B an's epididymal sperm concentration (1240 ± 35 × 10 6 /mL) was lower ( P < 0.01) compared with Landrace (4160 ± 42 × 10 6 /mL). However, sperm characteristics before and after freezing in B an and L andrace were similar. The numbers of follicles with diameter larger than 2 mm per ovary in B an females treated with equine chorionic gonadotropin and human chorionic gonadotropin (27.1 ± 1.3) were higher ( P < 0.05) than those in L andrace (12.9 ± 2.0) and in non‐hormone stimulated B an (no > 2 mm follicles). After in vitro maturation, the percentages of oocytes with expanded cumulus cells and the first polar body (matured oocytes) were not different among B an, hormone‐stimulated B an and L andrace. The percentages of two‐cell embryos and morulae derived from oocytes collected from three sources did not differ. However, the rate of blastocysts derived from oocytes in non‐stimulated B an (4.0 ± 3.8%) was lower ( P < 0.05) than that in Landrace (15.3 ± 1.8%). In conclusion, an effective IVP system for good quality embryos in B an, that is essential for genetic conservation of this breed, was established.