Premium
The growth‐promoting activity of egg white proteins in the C2C12 myoblast cell line
Author(s) -
Mizunoya Wataru,
Tashima Ayumi,
Sato Yusuke,
Tatsumi Ryuichi,
Ikeuchi Yoshihide
Publication year - 2015
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12257
Subject(s) - ovotransferrin , ovalbumin , c2c12 , egg white , myogenesis , chemistry , cell growth , lysozyme , myocyte , microbiology and biotechnology , biochemistry , biology , immunology , immune system
Abstract In this study, we examined the effects of several egg white proteins (ovalbumin, ovomucoid, ovotransferrin and lysozyme) on proliferation and myotube growth in C2C12 murine myoblast cells. Cell proliferation was measured using a water‐soluble tetrazolium salt ( WST ‐8)‐based assay and then validated using Giemsa staining. Significant proliferative activities of C2C12 cells were observed in response to the addition of 10 −5 –10 −4 mol/L ovalbumin or ovomucoid. Ovotransferrin decreased C2C12 cell proliferation and lysozyme showed no significant effects on the proliferation of C2C12 cells. In contrast, the proliferative effects of ovalbumin and ovomucoid were not observed in 3 T 3‐ L 1 murine preadipocyte cells. We also measured the effects of ovalbumin and ovomucoid on C2C12 myotube diameters by using histological analysis. In comparison to control cells, myotube diameters were significantly increased in cells cultured in 10 −6 –10 −4 mol/L ovalbumin or ovomucoid, suggesting that ovalbumin and ovomucoid stimulate the growth of myotubes. Thus, our results clearly demonstrated that ovalbumin or ovomucoid stimulated the proliferation of myoblasts and growth of myotubes.