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High‐level exogenous trans 10, cis 12 conjugated linoleic acid plays an anti‐lipogenesis role in bovine mammary epithelial cells
Author(s) -
Wang Hongfang,
Liu Hongyun,
Liu Jianxin,
Zhao Ke,
Wang Chong,
Yang Weiren
Publication year - 2014
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12204
Subject(s) - conjugated linoleic acid , lipogenesis , fatty acid synthase , chemistry , diacylglycerol kinase , acetyl coa carboxylase , linoleic acid , fatty acid , fatty acid synthesis , medicine , endocrinology , biochemistry , enzyme , metabolism , pyruvate carboxylase , biology , protein kinase c
Primary bovine mammary epithelial cells (BMECs) were treated by 0, 37.5, 75, 112.5, 150 μmol/L trans 10, cis 12 conjugated linoleic acid ( CLA ) to evaluate the effects of different level trans 10, cis 12 CLA on lipogenesis in BMEC . Addition of 75–150 μmol/L trans 10, cis 12 CLA reduced significantly the triacylglycerol ( TAG ) content ( P < 0.05), but did not have inhibiting action on cell proliferation ( P > 0.05). Treatment with 150 μmol/L trans 10, cis 12 CLA for 48 h resulted in a 17.1% reduction ( P < 0.0001) of medium chain fatty acids ( MCFA , C 14 < C < C 16), a 26.5% reduction ( P < 0.0001) of unsaturated fatty acids ( UFA ) and a corresponding reduction of the mRNA abundance of acetyl coenzyme A ( acetylCoA ) carboxylase ( ACC ) ( P = 0.046), fatty acid synthase ( FAS ) ( P = 0.017) and stearoylCoA desaturase1 ( SCD 1 ) ( P = 0.002). Another finding was that trans 10, cis 12 CLA elevated expression of diacylglycerol acyltransferase2 ( DGAT 2 ) ( P = 0.020) and long chain acylCoA synthetases ( ACSL ) ( P = 0.032). In conclusion, higher trans 10, cis 12 CLA , not low trans 10, cis 12 CLA , inhibited milk fat synthesis and changed fatty acid composition by regulating the expression of FAS , ACC , SCD 1, DGAT 2 and ACSL .