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Interaction of rumen bacteria as assumed by colonization patterns on untreated and alkali‐treated rice straw
Author(s) -
Koike Satoshi,
Yabuki Hiroyoshi,
Kobayashi Yasuo
Publication year - 2014
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12176
Subject(s) - rumen , colonization , straw , alkali metal , rice straw , bacteria , agronomy , chemistry , microbiology and biotechnology , zoology , biology , food science , fermentation , genetics , organic chemistry
Colonization patterns of representative rumen bacteria were compared between untreated rice straw ( UTS ) and sodium hydroxide‐treated rice straw ( SHTS ). UTS and SHTS were incubated in the rumen of sheep for 10 min, 1, 2, 6, 12, 24, 48 and 96 h using the nylon bag method. The population sizes of 13 representative bacterial species or groups were quantified by real‐time PCR . The total bacterial population size (abundance) was similar in both UTS and SHTS . F ibrobacter succinogenes showed a higher population size compared to other fibrolytic species and was detected at a higher level in SHTS (3.7%) than in UTS (2.6%). R uminococcus albus and Ruminococcus flavefaciens were also detected at higher levels in SHTS (0.15% and 0.29%) than in UTS (0.03% and 0.18%). Population sizes of non‐fibrolytic species, such as S elenomonas ruminantium , A naerovibrio lipolytica and S uccinivibrio dextrinosolvens were higher in UTS than in SHTS . Coefficient of determination ( r 2 ) on population changes between bacterial species or groups were higher in UTS than in SHTS , suggesting the necessity of stronger bacterial interactions for UTS digestion. Therefore, not only colonization of fibrolytic species, but also synergistic interactions between different bacterial species may be key to the ruminal digestion of rice straw.