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Shotgun proteomic analysis of porcine colostrum and mature milk
Author(s) -
Ogawa Shohei,
Tsukahara Takamitsu,
Nishibayashi Ryoichiro,
Nakatani Masako,
Okutani Mie,
Nakanishi Nobuo,
Ushida Kazunari,
Inoue Ryo
Publication year - 2014
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12165
Subject(s) - colostrum , passive immunity , biology , immune system , immunity , ingestion , shotgun proteomics , placenta , lactoferrin , food science , andrology , antibody , pregnancy , immunology , fetus , medicine , endocrinology , biochemistry , proteomics , genetics , gene
The epitheliochorial nature of the porcine placenta prevents the transfer of maternal immunity. Therefore, ingestion of the colostrum immediately after birth is crucial for neonatal piglets to acquire passive immunity from the sow. We performed a shotgun proteomic analysis of porcine milk to reveal in detail the protein composition of porcine milk. On the basis of the S wiss‐ P rot database, 113 and 118 proteins were identified in the porcine colostrum and mature milk, respectively, and 50 of these proteins were common to both samples. Some immune‐related proteins, including interleukin‐18 ( IL ‐18), were unique to the colostrum. The IL ‐18 concentration in the colostrum and mature milk of four sows was measured to validate the proteomic analysis, and IL ‐18 was only detected in the colostrum (191.0 ± 53.9 pg/mL) and not in mature milk. In addition, some proteins involved in primary defense, such as azurocidin, which has never been detected in any other mammal's milk, were also identified in the colostrum.