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Expression and in situ localization of GATA4 , 5 and 6 mRNAs in ovine conceptuses and uterine endometria during the peri‐implantation period
Author(s) -
Bai Hanako,
Sakurai Toshihiro,
Godkin James D.,
Imakawa Kazuhiko
Publication year - 2014
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12156
Subject(s) - conceptus , endoderm , gata2 , gata4 , biology , andrology , blastocyst , embryo , in situ hybridization , embryogenesis , endocrinology , messenger rna , medicine , microbiology and biotechnology , embryonic stem cell , gene expression , haematopoiesis , genetics , gene , pregnancy , fetus , stem cell
Abstract In vertebrates, six GATA transcription factors, GATA1 through GATA6 , have been identified and GATA1 ‐3 is known to be involved in hematopoietic developments, while GATA4 ‐6 play roles in cardiac and endoderm developments. Recently, we and others have found that GATA2 and GATA3 found in the trophectoderm plays a role in gene expression specific to this cell type, but GATA4 ‐6 have not been well characterized in early embryonic developments. Using quantitative polymerase chain reaction ( qPCR ) and in situ hybridization, we examined the expression of GATA4 , 5 and 6 messenger RNAs ( mRNAs ) in ovine conceptuses and uteri during the peri‐implantation period. In ovine conceptuses, GATA4 , 5 and 6 transcripts were present on days 15, 17 and 21 (day 0 = day of mating), and high GATA5 and 6 mRNAs were found on day 21, most of which were localized in the trophectoderm and endoderm. Moreover, minute and substantial GATA4 and 5 mRNAs were found in days 15 and 21 uterine endometria, respectively. Increase in GATA4 ‐6 transcripts in day 21 uteri indicates that in addition to GATA1 ‐3, GATA4 ‐6 may also play a potentially novel role in the development of ovine trophectoderm, endoderm and/or uterine endometria following conceptus attachment to the uterine epithelium.