Premium
Identification of novel genetic markers and evaluation of genetic structure in a population of J apanese crested ibis
Author(s) -
Tsubono Kanako,
Taniguchi Yukio,
Matsuda Hirokazu,
Yamada Takahisa,
Sugiyama Toshie,
Homma Kosuke,
Kaneko Yoshinori,
Yamagishi Satoshi,
Iwaisaki Hiroaki
Publication year - 2014
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12155
Subject(s) - microsatellite , genetic diversity , biology , genetics , genetic marker , primer (cosmetics) , genetic structure , population , allele , identification (biology) , evolutionary biology , genetic variation , gene , medicine , chemistry , environmental health , organic chemistry , botany
Japanese population of the J apanese crested ibis N ipponia nippon was founded by five individuals gifted from the P eople's R epublic of C hina. In order to exactly evaluate genetic structure, we first performed development of novel genetic makers using 89 microsatellite primer pairs of related species for cross‐amplification. Of these, only three primer pairs were useful for the genetic markers. Additionally, we sequenced allelic PCR products of these three markers together with 10 markers previously identified. Most markers showed typical microsatellite repeat units, but two markers were not simple microsatellites. Moreover, over half of the markers did not have the same repeat units as those of the original species. These results suggested that development of novel genetic markers in this population by cross‐amplification is not efficient, partly because of low genetic diversity. Furthermore, the cluster analysis by STRUCTURE program using 17 markers showed that the five founders were divided into two clusters. However, the genetic relationships among the founders indicated by the clustering seemed to be questionable, because the analysis relied largely on a small number of triallelic markers, in spite of the addition of the three useful markers. Therefore, more efficient methods for identifying large numbers of single nucleotide polymorphisms are desirable.