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Haplotype C of growth hormone ( GH ) gene in J apanese B lack cattle: Structure of GH protein and a novel method for detection of the gene
Author(s) -
Bahrami Abolfazl,
Ardiyanti Astrid,
Tonosaki Kaoru,
Suzuki Keiichi,
Tanida Nanako,
Hirayama Takuji,
Roh SangGun,
Nishio Takeshi,
Katoh Kazuo
Publication year - 2013
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12132
Subject(s) - single nucleotide polymorphism , receptor , chemistry , haplotype , gene , genotyping , biology , microbiology and biotechnology , biochemistry , genotype
From a series of studies on single nucleotide polymorphisms ( SNP s) in the bovine growth hormone ( GH ) gene of J apanese B lack cattle, the type‐ C (127 Val and 172 Met ) that is specific for this breed has been intensively focused upon because of the economic importance for carcass traits, such as intramuscular oleic acid contents. In the present study, we intended to analyze the 3‐ D structure of GH of haplotype C , and developed a novel method to detect the type C gene. Three‐ D analysis of the type C protein showed that the amino acid residues (127 Val and 172 Met ), which are present in the third and fourth helixes, respectively, and are important for binding with GH receptors, are shifted to deeper positions in the molecule compared with that for type A (127 Leu and 172 Thr ), implying the alteration of binding interaction with receptors. A novel, efficient and cost‐effective method ( D ot‐blot‐ SNP technique) for type C genotyping was successfully established, of which the basal method was a reported genotyping of SNP s for a large number of plants, reducing the cost to 10% or less of direct sequencing.