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Effect of bromochloromethane and fumarate on phylogenetic diversity of the formyltetrahydrofolate synthetase gene in bovine rumen
Author(s) -
Mitsumori Makoto,
Matsui Hiroki,
Tajima Kiyoshi,
Shinkai Takumi,
Takenaka Akio,
Denman Stuart E.,
McSweeney Christopher S.
Publication year - 2014
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12072
Subject(s) - rumen , phylogenetic tree , biology , bacteria , population , gene , biochemistry , microbiology and biotechnology , zoology , genetics , fermentation , demography , sociology
Effect of the methane inhibitor, bromochloromethane ( BCM ) and dietary substrate, fumarate, on microbial community structure of acetogen bacteria in the bovine rumen was investigated through analysis of the formyltetrahydrofolate synthetase gene ( fhs ). The fhs sequences obtained from BCM ‐untreated, BCM ‐treated, fumarate‐untreated and fumarate‐treated bovine rumen were categorized into homoacetogens and nonhomoacetogenic bacteria by homoacetogen similarity scores. Phylogenetic tree analysis indicated that most of the fhs sequences categorized into homoacetogens were divided into nine clusters, which were in close agreement with a result shown in a self‐organizing map. The diversity of the fhs sequences from the BCM ‐treated rumen was significantly different from those from BCM ‐non‐treated rumen. Principal component analysis also showed that addition of BCM to the rumen altered the population structure of acetogenic bacteria significantly but the effect of fumarate was comparatively minor. These results indicate that BCM affects diversity of actogens in the bovine rumen, and changes in acetogenic community structure in response to methane inhibitors may be caused by different mechanisms.