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Naloxone increases maturation rate and ratio of inner cell mass to total cells in blastocysts in pigs
Author(s) -
DangNguyen Thanh Quang,
Viet Linh Nguyen,
Minoia Rosa,
Kaneda Masahiro,
Somfai Tamas,
Haraguchi Seiki,
Akagi Satoshi,
Kikuchi Kazuhiro,
Nakai Michiko,
Tajima Atsushi,
Nagai Takashi
Publication year - 2013
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12071
Subject(s) - germinal vesicle , (+) naloxone , oocyte , endocrinology , medicine , in vitro maturation , chemistry , embryogenesis , inner cell mass , blastocyst , embryo , andrology , human fertilization , opioid , biology , receptor , anatomy , microbiology and biotechnology
The purposes of the present study were to examine the effect of naloxone, a mu‐opioid receptor ( MOR ) antagonist, on porcine oocyte maturation and embryo development. MOR gene was expressed in germinal vesicle ( GV ) and metaphase II ( M ‐ II ) porcine oocytes, one‐, four‐cell stage embryos and blastocysts. In blastocysts, MOR gene was mainly expressed in inner cell mass ( ICM ) cells. Supplementation of 10 −8 mol/L naloxone in in vitro maturation ( IVM ) medium increased the maturation rate ( P < 0.05). However, 10 −4 mol/L naloxone reduced the maturation rate ( P < 0.05) compared with the control. The presence of naloxone during IVM had no effects on fertilization status and subsequent embryonic development after in vitro culture ( IVC ). The addition of 10 −3 mol/L dibutyryl cyclic adenosine monophosphate ( dbcAMP ), and 10 −8 mol/L naloxone together into IVM medium increased nuclear maturation ( P < 0.05) compared with the addition of either dbcAMP or naloxone alone. Supplementation with naloxone in IVC medium did not improve embryonic development. However, at the concentrations of 10 −6 mol/L and 10 −8 mol/L, naloxone increased the ratio of ICM to total cells in blastocysts ( P < 0.05). In conclusion, at low concentration, naloxone increases maturation rate and the ratio of ICM to total cells in blastocysts. Naloxone and cAMP have a synergistic effect on oocyte maturation.