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Vitrification procedure decreases inositol 1,4,5‐trisphophate receptor expression, resulting in low fertility of pig oocytes
Author(s) -
Hirose Masahiko,
Kamoshita Maki,
Fujiwara Katsuyoshi,
Kato Tsubasa,
Nakamura Ayaka,
Wojcikiewicz Richard J. H.,
Parys Jan B.,
Ito Junya,
Kashiwazaki Naomi
Publication year - 2013
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12061
Subject(s) - vitrification , andrology , cryopreservation , oocyte , human fertilization , in vitro fertilisation , fertility preservation , in vitro maturation , fertility , embryo , biology , oocyte cryopreservation , chemistry , microbiology and biotechnology , anatomy , medicine , population , environmental health
Although cryopreservation of mammalian oocytes is an important technology, it is well known that unfertilized oocytes, especially in pigs, are highly sensitive to low temperature and that cryopreserved oocytes show low fertility and developmental ability. The aim of the present study was to clarify why porcine in vitro matured ( IVM ) oocytes at the metaphase II ( MII ) stage showed low fertility and developmental ability after vitrification. In vitro matured cumulus oocyte complexes ( COCs ) were vitrified with C ryotop and then evaluated for fertility through in vitro fertilization ( IVF ). Although sperm‐penetrated oocytes were observed to some extent (30–40%), the rate of pronuclear formation was low (9%) and none of them progressed to the two‐cell stage. The results suggest that activation ability of cryopreserved oocytes was decreased by vitrification. We examined the localization and expression level of the type 1 inositol 1,4,5 trisphosphate receptor ( IP 3 R1 ), the channel responsible for Ca 2+ release during IVF in porcine oocytes. Localization of IP 3 R 1 close to the plasma membrane and total expression level of IP 3 R1 protein were both decreased by vitrification. In conclusion, our present study indicates that vitrified‐warmed porcine COCs showed a high survival rate but low fertility after IVF . This low fertility seems to be due to the decrease in IP 3 R1 by the vitrification procedure.