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Effect of synchronization of donor cells in early G 1‐phase using shake‐off method on developmental potential of somatic cell nuclear transfer embryos in cattle
Author(s) -
Goto Yuji,
Hirayama Muneyuki,
Takeda Kazuya,
Tukamoto Nobuyuki,
Sakata Osamu,
Kaeriyama Hiroshi,
Geshi Masaya
Publication year - 2013
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12047
Subject(s) - somatic cell nuclear transfer , andrology , embryo , biology , blastocyst , somatic cell , cell synchronization , microbiology and biotechnology , embryogenesis , genetics , cell , cell cycle , gene , medicine
In this study, we compared the developmental ability of somatic cell nuclear transfer ( SCNT ) embryos reconstructed with three bovine somatic cells that had been synchronized in G 0‐phase ( G 0‐ SCNT group) or early G 1‐phase (e G 1‐ SCNT group). Furthermore, we investigated the production efficiency of cloned offspring for NT embryos derived from these donor cells. The G 0‐phase and e G 1‐phase cells were synchronized, respectively, using serum starvation and antimitotic reagent treatment combined with shaking of the plate containing the cells (shake‐off method). The fusion rate in the G 0‐ SCNT groups (64.2 ± 1.8%) was significantly higher than that of e G 1‐ SCNT groups (39.2 ± 1.9%) ( P < 0.05), but the developmental rates to the blastocyst stage of SCNT embryos per fused oocytes were similar for all groups. The overall production efficiency of the clone offspring in e G 1‐ SCNT groups (12.7%) per recipient cow was higher than that in G 0‐ SCNT groups (3%) ( P < 0.05). The mean birth weight of cloned calves and the average calving score in the G 0‐ SCNT groups (48.1 ± 3.4 kg and 3.3 ± 0.3, respectively) was significantly higher ( P < 0.05) than those of e G 1‐ SCNT groups (37.2 ± 2.1 kg and 2.3 ± 0.2, respectively). Results of this study indicate that synchronization of donor cells in e G 1‐phase using the shake‐off method improved the overall production efficiency of the clone offspring per transferred embryo.