Premium
Cloning, tissue expression and polymorphisms of chicken K rüppel‐like factor 7 gene
Author(s) -
Zhang ZhiWei,
Wang ZhiPeng,
Zhang Kun,
Wang Ning,
Li Hui
Publication year - 2013
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12043
Subject(s) - broiler , biology , cloning (programming) , single nucleotide polymorphism , gene , exon , locus (genetics) , genetics , population , coding region , microbiology and biotechnology , genotype , zoology , demography , sociology , computer science , programming language
K rüppel‐like factor 7 ( KLF7 ) has been extensively studied in mammalian species, but its role in birds is still unclear. In the current study, cloning and sequencing showed that the full‐length coding region of chicken KLF7 ( G allus gallus KLF7 , gKLF7 ) was 891 bp long, encoding 296 amino acids. In addition, real‐time RT‐PCR analysis showed that gKLF7 was broadly expressed in all 15 chicken tissues selected, and its expression was significantly different in spleen, proventriculus, abdominal fat, brain, leg muscle, gizzard and heart between fat and lean broilers at 7 weeks of age. Additionally, one novel single nucleotide polymorphism ( SNP ), XM _426569.3: c. A141G , was identified in the second exon of gKLF7 . Association analysis showed that this locus was significantly associated with fatness traits in Arbor Acres broiler random population and the eighth generation of Northeast Agricultural University broiler lines divergently selected for abdominal fat content ( NEAUHLF ) population ( P < 0.05). These results suggest that gKLF7 might be a candidate gene for chicken fatness traits.