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Satellite cells produce neural chemorepellent semaphorin 3A upon muscle injury
Author(s) -
SATO Yusuke,
DO MaiKhoi Q.,
SUZUKI Takahiro,
OHTSUBO Hideaki,
MIZUNOYA Wataru,
NAKAMURA Mako,
FURUSE Mitsuhiro,
IKEUCHI Yoshihide,
TATSUMI Ryuichi
Publication year - 2013
Publication title -
animal science journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.606
H-Index - 38
eISSN - 1740-0929
pISSN - 1344-3941
DOI - 10.1111/asj.12014
Subject(s) - semaphorin , sema3a , microbiology and biotechnology , myod , myogenin , myogenesis , neuroscience , satellite , biology , myocyte , receptor , biochemistry , aerospace engineering , engineering
Regenerative mechanisms that regulate intramuscular motor innervation. including configuration of the neuromuscular connections are thought to reside in the spatiotemporal expression of axon‐guidance molecules. Our previous studies proposed a heretofore unexplored role of satellite cells as a key source of a secreted neural chemorepellent semaphorin 3A ( Sema3A ) expression. In order to verify this concept, there is still a critical need to provide direct evidence to show the up‐regulation of Sema3A protein in satellite cells in vivo upon muscle injury. The present study employed a Sema3A / MyoD double‐immunohistochemical staining for cryo‐sections prepared from cardiotoxin injected gastrocnemius muscle of adult mouse lower hind‐limb. Results clearly demonstrated that Sema3A expression was up‐regulated in myogenic differentiation‐positive satellite cells at 4–12 days post‐injury period, the time that corresponds to the cell differentiation phase characterized by increasing myogenin messenger RNA expression. This direct proof encourages a possible implication of satellite cells in the spatiotemporal regulation of extracellular Sema3A concentrations, which potentially ensures coordinating a delay in neurite sprouting and re‐attachment of motoneuron terminals onto damaged muscle fibers early in muscle regeneration in synchrony with recovery of muscle‐fiber integrity.