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Dietary Acanthopanax senticosus extracts modulated the inflammatory and apoptotic responses of yellow catfish to protect against Edwardsiella ictaluri infection
Author(s) -
Song Zixi,
Jiao Congrui,
Chen Baoyang,
Xu Wenyu,
Wang Mengya,
Zou Jiahong,
Xu Weiqi,
Xu Zhen,
Wang Qingchao
Publication year - 2021
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.15379
Subject(s) - edwardsiella ictaluri , catfish , biology , apoptosis , necrosis , spleen , microbiology and biotechnology , andrology , immunology , fish <actinopterygii> , fishery , biochemistry , medicine , genetics , ictalurus
Herbal extracts interfere with bacterial adhesion and support apoptosis‐dependent exfoliation to protect mammals against bacterial infection. In this study, juvenile yellow catfish were fed with control diet or Acanthopanax senticosus extracts (AS)‐supplemented diet. After 60 days, AS (180 ml/kg feed) significantly elevated fish growth and feed utilization, with higher weight gain and feed efficiency. After challenged with Edwardsiella ictaluri , survival rate of AS‐supplemented fish (65%) was significantly higher than control fish (30%). Control fish exhibited obvious skin lesions since 1 day post‐infection (dpi) and decreased thickness of epidermis with prolonged days, while AS‐supplemented fish skin structure showed better integrity. Moreover, length of secondary branchial filaments of AS‐supplemented fish was higher than control fish that showed obvious occlusion between adjacent lamellae. Unlike its pro‐inflammatory role in spleen, AS significantly inhibited gill inflammation at 4 dpi via regulating TLRs‐NFκB pathway. Moreover, haematoxylin–eosin staining exhibited that AS delayed the liver vasculitis and necrotic hepatitis of yellow catfish. Further study by TUNEL assay, caspase‐3 activity and mRNA expression indicated that AS significantly promoted hepatic apoptosis at 1 dpi to prevent further necrosis, which process was regulated by JAK/STAT pathway. All results demonstrated AS modulated inflammation and apoptosis to protect yellow catfish against Edwardsiella ictaluri infection.