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Using droplet digital PCR to detect plant DNA in tissues of zebrafish ( Danio rerio ) fed genetically modified maize
Author(s) -
Dong Shanshan,
Zhang Dini,
Yu Cigang,
Zhang Zhenhua,
Liu Yan
Publication year - 2021
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.15285
Subject(s) - danio , biology , genetically modified organism , zebrafish , transgene , digital polymerase chain reaction , exogenous dna , dna , genetically modified maize , polymerase chain reaction , genetically modified crops , endogeny , microbiology and biotechnology , gene , genetics , biochemistry
The global commercialization of genetically modified (GM) crops has increased the likelihood of feed‐derived transgenic DNA uptake and raised concerns regarding the fate of such DNA fragments in animals consuming a GM diet. The present study applied a novel droplet digital polymerase chain reaction (ddPCR) method to quantitatively detect the concentrations of feed‐derived transgenic ( Cry1Ie ) and endogenous ( rubisco ) plant DNA in the livers, intestinal tracts and muscles of zebrafish ( Danio rerio ) fed GM maize for uantifying feed‐derived DNA in animal tissues as a more sensitive and reliable alternative to qPCR.