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Regulation of gene expression associated with LC‐PUFA metabolism in juvenile tambaqui ( Colossoma macropomum ) fed different dietary oil sources
Author(s) -
Ferraz Renato B.,
Modesto André L.S.,
Cunha Isabel,
Ozório Rodrigo,
O´Sullivan Fernanda L.A.,
Salaro Ana L.,
Monroig Óscar,
Castro L. Filipe C.
Publication year - 2021
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.15236
Subject(s) - tambaqui , biology , fads2 , polyunsaturated fatty acid , linseed oil , food science , fish oil , aquaculture , nannochloropsis , biochemistry , botany , fatty acid , docosahexaenoic acid , fish <actinopterygii> , fishery , algae
Given the ecological unsustainability of using fish oil (FO) as the main source of long‐chain polyunsaturated fatty acids (LC‐PUFA), the investigation of alternative dietary LC‐PUFA sources is crucial for aquaculture. Tambaqui ( Colossoma macropomum ) is a valuable economic aquaculture resource in Brazil, capable of endogenously elongating and desaturating linoleic (LA; 18:2n‐6) and α‐linolenic (ALA; 18:3n‐3) acids to longer and physiologically vital LC‐PUFA. Yet, it is unclear how this pathway is regulated by different oil sources in tambaqui. Thus, we designed an experiment with 2 different oil diets (fish oil—FO and vegetable oil—VO) at 2 different concentrations (5% or 10%) to investigate the molecular regulation of fatty acid biosynthesis in this species. We observed no differences in survival, body weight gain (BWG) and feed conversion ratio between the treatments. Gene expression analysis shows that fads2 and elovl5 are up‐regulated in liver, while fads2 and elovl2 are up‐regulated in brain of tambaqui fed with VO. The transcription factors pparβb and pparγ are also up‐regulated in the brain by VO diet, when compared to FO diet. The VO diet also contributed to the biosynthesis of LC‐PUFA in liver and specifically DHA in the brain. Overall, our approach shows that lipid metabolism‐relevant genes are regulated by different dietary lipid sources.