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Immune responses and protective efficacy of diet supplementation with selenium nanoparticles against cadmium toxicity in Oreochromis niloticus
Author(s) -
AbuElala Nermeen M.,
Shaalan Mohamed,
Ali Sara E.,
Younis Nehal A.
Publication year - 2021
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.15212
Subject(s) - oreochromis , biology , malondialdehyde , catalase , toxicity , nile tilapia , immune system , glutathione , antioxidant , glutathione peroxidase , tumor necrosis factor alpha , endocrinology , medicine , immunology , oxidative stress , biochemistry , enzyme , fishery , fish <actinopterygii>
The present study aims to determine the effects of dietary selenium nanoparticles (Se‐NPs) on growth, immune response and histopathological alterations induced by sublethal cadmium (Cd) toxicity in Nile tilapia ( Oreochromis niloticus ). It was carried out through two phases: in the first phase, fish were divided into two groups: a control group fed a basal diet and a treated group fed Se‐NP‐supplemented diet at 1 mg/kg for four weeks. Growth indices, innate immunological parameters and gene expression of interleukin ( IL ‐ 1β ), tumour necrosis factor ( TNF ‐ α ), catalase ( CAT ) and insulin growth factor ( IGF ‐ 1 ) were measured. In the second phase, fish were subdivided into four groups: G1, control, G2 was fed a control diet and exposed to Cd (5 mg/L water), G3 was fed Se‐NPs‐supplemented diet and G4 was fed Se‐NPs‐supplemented diet and exposed to Cd (5 mg/L water). Tissue samples were collected for histopathological investigation and measuring of malondialdehyde (MDA) and glutathione reduced (GSH) activity 10 days post‐Cd exposure. Dietary Se‐NPs increased phagocytic and lysozyme activities in the 2 nd week, whereas the phagocytic index and growth indices were increased in the 4 th week. IGF ‐ 1 was upregulated after 2 weeks, and TNF ‐ α , IL ‐ 1β and CAT genes upregulated after 4 weeks. Cd caused gill hyperplasia and degenerative changes in visceral organs, elevation in MDA and attenuation in GSH activity. However, in G4 group, the histopathological alterations and antioxidant enzyme were alleviated. Thus, we conclude that dietary Se‐NPs improve the growth, immunity and antioxidant power of O . niloticus and alleviate the pathological disorders induced by Cd toxicity.