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GC–MS‐based metabolomics reveals metabolic changes in overwintering scylla paramamosain at two different salinities
Author(s) -
Zhou Junming,
Li Na,
Fu Yuanyuan,
He Congying,
Meng Kuanhong,
Li Yanrong,
Zhu Zhujun,
Huang Lili,
Hu Daifu,
Wang Chunlin,
Liu Lei
Publication year - 2021
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.15157
Subject(s) - scylla paramamosain , overwintering , biology , salinity , botany , biochemistry , isoleucine , food science , amino acid , ecology , leucine , gene
Overwintering is an important part of the life cycle of Scylla paramamosain . Salinity has a significant effect on the growth and metabolism of overwintering S . paramamosain . 4‰ and 25‰ are typical salinities in different regions where S. paramamosain overwinters. In order to find the optimum salinity for overwintering, the metabolites in overwintering S. paramamosain at salinities of 4‰ and 25‰ were analysed and compared using GC–MS technology. In this study, the weight gain rate of S . paramamosain was 5.51% at 4‰ salinity and 10.69% at 25‰ salinity after overwintering. A total of 1254 valid peaks and 373 metabolites were detected, including 160 significantly different metabolites (16 down‐regulated metabolites and 144 up‐regulated metabolites). In the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, 22 pathways were associated with metabolic changes; they have or identified L‐isoleucine, L‐lysine, urea, D‐ribose‐5‐phosphate, glucose‐6‐phosphate, fumaric acid, L‐lactic acid, docosahexaenoic acid and palmitic acid. Compared with the 25‰ salinity group, 4‰ salinity group promoted amino acid metabolism, carbohydrate metabolism, membrane transport and lipid metabolism through these differential metabolites, so that overwintering S. paramamosain needs to participate in more metabolic activities at 4‰ salinity, and hepatopancreas consumes more energy to maintain life activities, which indicated that 4‰ salinity was not conducive to the overwintering of S. paramamosain .