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Knockdown of igf3 gene by RNA interference causes gonadal development inhibition of common carp ( Cyprinus carpio )
Author(s) -
Song Feibiao,
Wang Lanmei,
Yu Zhenzhen,
Zhu Wenbin,
Fu Jianjun,
Dong Zaijie
Publication year - 2021
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/are.15145
Subject(s) - gene knockdown , biology , rna interference , cyprinus , development of the gonads , carp , hypothalamic–pituitary–gonadal axis , gonad , endocrinology , medicine , gene , rna silencing , hormone , ovary , andrology , microbiology and biotechnology , rna , fish <actinopterygii> , fishery , genetics , luteinizing hormone
RNA interference is an important method in studying the gene function of organisms. As a novel important gene in gonadal development of teleost fish, the function of igf3 is worth of researching. In this study, the igf3b ‐dsRNA of C. carpio was successfully synthesized, and long‐term igf3 interference experiments were conducted. Intraperitoneal injection of igf3b ‐dsRNA could influence the serum sex hormone level and significantly reduced the expression level of insulin‐like growth factors (IGFs) in hypothalamic–pituitary–gonad (HPG) axis. After a 60‐day interference experiment, igf3 knockdown could affect the development of ovary and testis of juvenile C. carpio . These findings show that igf3 knockdown may have a negative effect on gonadal development of C. carpio through HPG axis.